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Humain AK4 / Adenylate Kinase 4 / AK3L1 Gène ADNc clone le vecteur de clonage

Fiche techniqueCommentairesProduits apparentésProtocoles
Human AK4/AK3L1 Informations sur les produits clonés de cDNA
Gene_bank_ref_id:BC016180
Taille du ADNc:672bp
Description du ADNc:Full length Clone DNA of Homo sapiens adenylate kinase 4.
Synonyme du gène:AK3, AK3L1, AK3L2
Espèces:Human
Vecteur:pGEM-T Vector
Plasmid:pGEM-AK3L1
Site de restriction:
Séquence du marqueur:
Description de la séquence:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Stockage:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Humain AK4 / Adenylate Kinase 4 / AK3L1 Gène ADNc clone le vecteur de clonage on other vectors
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurHG12406-ACGCHF270
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurHG12406-ACRCHF270
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, N-GFPSpark MarqueurHG12406-ANGCHF270
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, N-OFPSpark MarqueurHG12406-ANRCHF270
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG12406-CFCHF230
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurHG12406-CHCHF230
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurHG12406-CMCHF230
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurHG12406-CYCHF230
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurHG12406-NFCHF230
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurHG12406-NHCHF230
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurHG12406-NMCHF230
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurHG12406-NYCHF230
Humain AK4 / Adenylate Kinase 4 / AK3L1 expression plasmide de Gène l'ADNc ORF cloneHG12406-UTCHF230
 En savoir plus sur les vecteurs d'expression
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Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

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Catalogue : HG12406-G
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