Carbonic Anhydrase VIII/CA8 Matched ELISA Antibody Pair Set,Mouse

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Carbonic Anhydrase VIII/CA8 Matched ELISA Antibody Pair Set,Mouse General Information

Product name
Carbonic Anhydrase VIII/CA8 Matched ELISA Antibody Pair Set,Mouse
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Mouse Carbonic Anhydrase VIII/CA8
Assay range
31.25-2000 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse Carbonic Anhydrase VIII/CA8 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50767-R057)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse Carbonic Anhydrase VIII/CA8 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50767-R001)
3. Standard Each vial contains 98 ng of recombinant Mouse Carbonic Anhydrase VIII/CA8. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
Product overview
This Carbonic Anhydrase VIII/CA8 Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse Carbonic Anhydrase VIII/CA8 . It contains Mouse Carbonic Anhydrase VIII/CA8 capture antibody, Mouse Carbonic Anhydrase VIII/CA8 detector antibody (HRP) and a highly purified E. coli-expressed recombinant Mouse Carbonic Anhydrase VIII/CA8 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Carbonic Anhydrase VIII/CA8 Matched ELISA Antibody Pair Set,Mouse Images

Carbonic Anhydrase VIII/CA8 Matched ELISA Antibody Pair Set,Mouse: Alternative Names

AW546993 Matched ELISA Antibody Pair Set, Mouse; Ca8 Matched ELISA Antibody Pair Set, Mouse; Cals Matched ELISA Antibody Pair Set, Mouse; Cals1 Matched ELISA Antibody Pair Set, Mouse; Car8 Matched ELISA Antibody Pair Set, Mouse; Carp Matched ELISA Antibody Pair Set, Mouse; RP23-180H12.1 Matched ELISA Antibody Pair Set, Mouse; wdl Matched ELISA Antibody Pair Set, Mouse

Carbonic Anhydrase VIII/CA8 Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Full Name
carbonic anhydrase VIII
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
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