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Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, N-Myc Marqueur

Fiche techniqueCommentairesProduits apparentésProtocoles
Cynomolgus ENSA Informations sur les produits clonés de cDNA
Gene_bank_ref_id:XM_005541982.1
Taille du ADNc:366bp
Description du ADNc:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) endosulfine alpha with N terminal Myc tag.
Synonyme du gène:ENSA
Espèces:Cynomolgus
Vecteur:pCMV3-N-Myc
Plasmid:
Site de restriction:
Séquence du marqueur:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Description de la séquence:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Stockage:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, N-Myc Marqueur on other vectors
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurCG90656-ACGCHF270
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurCG90656-ACRCHF270
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, N-GFPSpark MarqueurCG90656-ANGCHF270
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, N-OFPSpark MarqueurCG90656-ANRCHF270
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurCG90656-CFCHF234
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurCG90656-CHCHF234
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurCG90656-CMCHF234
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurCG90656-CYCHF234
Macaque de Buffon ENSA / Endosulfine alpha Gène ADNc clone le vecteur de clonageCG90656-GCHF90
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurCG90656-NFCHF234
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurCG90656-NHCHF234
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurCG90656-NMCHF234
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurCG90656-NYCHF234
Macaque de Buffon ENSA / Endosulfine alpha expression plasmide de Gène l'ADNc ORF cloneCG90656-UTCHF234
 En savoir plus sur les vecteurs d'expression
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Fond

Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

Références
  • Ye M. et al., 2001, Genome Res. 10 (10): 1546-60.
  • Apiou F. et al., 1999, Diabetes 48 (9): 1873-6.
  • Lennon G. et al., 1997, Genome Res. 6 (9): 791-806.
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    Catalogue : CG90656-NM
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