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Macaque de Buffon EF1B / EEF1B2 Gène ADNc clone le vecteur de clonage

Fiche techniqueCommentairesProduits apparentésProtocoles
Cynomolgus EEF1B2 Informations sur les produits clonés de cDNA
Gene_bank_ref_id:NM_001284704.1
Taille du ADNc:678bp
Description du ADNc:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) eukaryotic translation elongation factor 1 beta 2.
Synonyme du gène:EEF1B2
Espèces:Cynomolgus
Vecteur:pGEM-T Vector
Plasmid:pGEM-cynoEEF1B2
Site de restriction:
Séquence du marqueur:
Description de la séquence:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 210G/A, 579C/T not causing the amino acid variation. Please check the sequence information before order.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Stockage:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Macaque de Buffon EF1B / EEF1B2 Gène ADNc clone le vecteur de clonage on other vectors
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurCG90457-ACGCHF270
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurCG90457-ACRCHF270
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, N-GFPSpark MarqueurCG90457-ANGCHF270
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, N-OFPSpark MarqueurCG90457-ANRCHF270
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurCG90457-CFCHF230
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurCG90457-CHCHF230
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurCG90457-CMCHF230
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurCG90457-CYCHF230
Macaque de Buffon EF1B / EEF1B2 Gène ADNc clone le vecteur de clonageCG90457-GCHF90
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurCG90457-NFCHF230
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurCG90457-NHCHF230
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurCG90457-NMCHF230
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurCG90457-NYCHF230
Macaque de Buffon EF1B / EEF1B2 expression plasmide de Gène l'ADNc ORF cloneCG90457-UTCHF230
 En savoir plus sur les vecteurs d'expression
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EF1B, also known as EEF1B2, is a translation elongation factor. It belongs to the EF-1-beta/EF-1-delta family. Elongation factors are a set of proteins that are used in protein synthesis in the cell. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. EF1B is more complex in eukaryotes than in bacteria, and consists of three subunits: EF1B-alpha, EF1B-gamma and EF1B-beta. EF1B contains 1 GST C-terminal domain. It is involved in the transfer of aminoacylated tRNAs to the ribosome. EF1B is required to regenerate EF1A from its inactive form (EF1A-GDP) to its active form (EF1A-GTP). EF1A is then ready to interact with a new aminoacyl-tRNA to begin the cycle again.

Références
  • Pizzuti A. et al., 1994, Biochem Biophys Res Commun. 197 (1): 154-62.
  • Rual. et al., 2005, Nature. 437 (7062): 1173-8.
  • Stelzl. et al., 2005, Cell. 122 (6): 957-68.
  • Sang Lee. et al., 2002, Biochem Biophys Res Commun. 291 (1): 158-64.
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