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Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, C-Flag Marqueur

Fiche techniqueCommentairesProduits apparentésProtocoles
Human ADH1A Informations sur les produits clonés de cDNA
Gene_bank_ref_id:BC117442
Taille du ADNc:1128bp
Description du ADNc:Full length Clone DNA of Homo sapiens alcohol dehydrogenase 1A (class I), alpha polypeptide with C terminal Flag tag.
Synonyme du gène:ADH1
Espèces:Human
Vecteur:pCMV3-C-FLAG
Plasmid:
Site de restriction:
Séquence du marqueur:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Description de la séquence:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Stockage:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, C-Flag Marqueur on other vectors
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurHG13937-ACGCHF270
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurHG13937-ACRCHF270
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, N-GFPSpark MarqueurHG13937-ANGCHF270
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, N-OFPSpark MarqueurHG13937-ANRCHF270
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG13937-CFCHF230
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurHG13937-CHCHF230
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurHG13937-CMCHF230
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurHG13937-CYCHF230
Humain alcohol dehydrogenase 1A/ADH1A Gène ADNc clone le vecteur de clonageHG13937-GCHF90
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurHG13937-NFCHF230
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurHG13937-NHCHF230
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurHG13937-NMCHF230
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurHG13937-NYCHF230
Humain alcohol dehydrogenase 1A/ADH1A expression plasmide de Gène l'ADNc ORF cloneHG13937-UTCHF230
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Catalogue : HG13937-CF
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