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Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Flag Marqueur

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Human BCL2A1 Informations sur les produits clonés de cDNA
Gene_bank_ref_id:NM_004049.3
Taille du ADNc:528bp
Description du ADNc:Full length Clone DNA of Homo sapiens BCL2-related protein A1,transcript variant 1 with N terminal Flag tag.
Synonyme du gène:GRS, BFL1, ACC-1, ACC-2, HBPA1, BCL2L5
Espèces:Human
Vecteur:pCMV3-N-FLAG
Plasmid:
Site de restriction:
Séquence du marqueur:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Description de la séquence:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Stockage:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Flag Marqueur on other vectors
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurHG10562-ACGCHF270
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurHG10562-ACRCHF270
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-GFPSpark MarqueurHG10562-ANGCHF270
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-OFPSpark MarqueurHG10562-ANRCHF270
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10562-CFCHF230
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurHG10562-CHCHF230
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurHG10562-CMCHF230
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurHG10562-CYCHF230
Humain Bcl2A1/Bfl-1 transcript variant 1 Gène ADNc clone le vecteur de clonageHG10562-MCHF90
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10562-M-FCHF230
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurHG10562-NFCHF230
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurHG10562-NHCHF230
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurHG10562-NMCHF230
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurHG10562-NYCHF230
Humain Bcl2A1/Bfl-1 transcript variant 1 expression plasmide de Gène l'ADNc ORF cloneHG10562-UTCHF230
 En savoir plus sur les vecteurs d'expression
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Catalogue : HG10562-NF
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