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Humain SPINLW1 expression plasmide de Gène l'ADNc ORF clone, N-Myc Marqueur

    Fiche techniqueCommentairesProduits apparentésProtocoles
    Humain EPPIN Informations sur les produits clonés de cDNA
    Gene_bank_ref_id:BC053369
    Taille du ADNc:402bp
    Description du ADNc:Full length Clone DNA of Homo sapiens serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin) with N terminal Myc tag.
    Synonyme du gène:CT71, CT72, WAP7, EPPIN, WFDC7, dJ461P17.2, SPINLW1
    Espèces:Human
    Vecteur:pCMV3-SP-N-Myc
    Plasmid:
    Site de restriction:
    Séquence du marqueur:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
    Description de la séquence:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with EPPIN qPCR primers for gene expression analysis, HP102419 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Stockage:The lyophilized plasmid can be stored at room temperature for three months.
    Myc Tag Info

    A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

    The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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    Catalogue : HG13742-NM
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