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Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, C-His Marqueur

Fiche techniqueCommentairesProduits apparentésProtocoles
Human FAP Informations sur les produits clonés de cDNA
Gene_bank_ref_id:NM_004460.2
Taille du ADNc:2283bp
Description du ADNc:Full length Clone DNA of Homo sapiens fibroblast activation protein, alpha with C terminal His tag.
Synonyme du gène:FAPA, DPPIV, DKFZp686G13158, FAP
Espèces:Human
Vecteur:pCMV3-C-His
Plasmid:
Site de restriction:
Séquence du marqueur:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Description de la séquence:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Stockage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, C-His Marqueur on other vectors
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurHG10464-ACGCHF290
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurHG10464-ACRCHF290
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10464-CFCHF260
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurHG10464-CHCHF260
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurHG10464-CMCHF260
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurHG10464-CYCHF260
Humain Fibroblast Activation Protéine alpha/FAP Gène ADNc clone le vecteur de clonageHG10464-MCHF90
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurHG10464-M-HCHF260
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurHG10464-NFCHF260
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurHG10464-NHCHF260
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurHG10464-NMCHF260
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurHG10464-NYCHF260
Humain Fibroblast Activation Protéine alpha/FAP expression plasmide de Gène l'ADNc ORF cloneHG10464-UTCHF260
 En savoir plus sur les vecteurs d'expression
Product nameProduct name
Fond

Seprase, also known as 170 kDa melanoma membrane-bound gelatinase , Fibroblast activation protein alpha, Integral membrane serine protease and FAP, is a single-pass type II membrane protein which belongs to the peptidase S9B family. Seprase / FAP is found in cell surface lamellipodia, invadopodia and on shed vesicles. Seprase / FAP appears to act as a proteolytically active 170-kDa dimer, consisting of two 97-kDa subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV ( DPPIV / CD26 ) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. Seprase / FAP colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Seprase / FAP colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. DPP4 and seprase exhibit multiple functions due to their abilities to form complexes with each other and to interact with other membrane-associated molecules. In association with DPP4, Seprase / FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. Seprase / FAP has a dual function in tumour progression. The proteolytic activity of Seprase has been shown to promote cell invasiveness towards the ECM and also to support tumour growth and proliferation. Seprase / FAP may have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

Références
  • Mori,Y. et al., 2004, Oncology. 67 (5-6):411-9.
  • Aertgeerts K., et al., 2005, J. Biol. Chem. 280:19441-19444.
  • Liu T., et al., 2005, J. Proteome Res. 4:2070-2080.
  • Ghersi G., et al., 2006, Cancer Res. 66:4652-4661.
  • O'Brien,P. et al., 2008, Biochim Biophys Acta. 1784 (9):1130-45.
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    Catalogue : HG10464-CH
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