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Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, N-Myc Marqueur

    Fiche techniqueCommentairesProduits apparentésProtocoles
    Humain MAPK8 Informations sur les produits clonés de cDNA
    Gene_bank_ref_id:NM_139047.1
    Taille du ADNc:1284bp
    Description du ADNc:Full length Clone DNA of Homo sapiens mitogen-activated protein kinase 8, transcript variant JNK1-b2 with N terminal Myc tag.
    Synonyme du gène:JNK, JNK1, PRKM8, SAPK1, JNK1A2, JNK21B1/2
    Espèces:Human
    Vecteur:pCMV3-N-Myc
    Plasmid:
    Site de restriction:
    Séquence du marqueur:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
    Description de la séquence:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with MAPK8 qPCR primers for gene expression analysis, HP100709 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Stockage:The lyophilized plasmid can be stored at room temperature for three months.
    Myc Tag Info

    A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

    The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, N-Myc Marqueur on other vectors
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurHG10795-ACGCHF270
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurHG10795-ACRCHF270
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, N-GFPSpark MarqueurHG10795-ANGCHF270
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, N-OFPSpark MarqueurHG10795-ANRCHF270
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10795-CFCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurHG10795-CHCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurHG10795-CMCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurHG10795-CYCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 Gène ADNc clone le vecteur de clonageHG10795-MCHF90
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10795-M-FCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF cloneHG10795-M-NCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurHG10795-NFCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurHG10795-NHCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurHG10795-NMCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurHG10795-NYCHF230
    Humain JNK1 / MAPK8 transcript variant JNK1-b2 expression plasmide de Gène l'ADNc ORF cloneHG10795-UTCHF230
     En savoir plus sur les vecteurs d'expression
    Product nameProduct name
    Fond

    Mitogen-activated protein kinase 8 (MAPK8), also known as JNK1, is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The protein kinases JNK1 has been found to serve as critical molecular links between obesity, metabolic inflammation, and disorders of glucose homeostasis. It is critically involved in the promotion of diet-induced obesity, metabolic inflammation and beta-cell dysfunction. The selective deficiency of JNK1 in the murine nervous system is sufficient to suppress diet-induced obesity. Genetic analysis indicates that the effects of JNK1 can be separated from effects of JNK1 on obesity. JNK1 is a potential pharmacological target for the development of drugs that might be useful for the treatment of metabolic syndrome, and type 2 diabetes. Furthermore, JNK1 plays a major role in the hypoxic cellular damage. JNK1 protein might be an attractive target for antihypoxic therapy in increasing resistance to many pathological conditions and diseases, leading to the oxygen deficit.

    Références
  • Betigeri S, et al. (2006) JNK1 as a molecular target to limit cellular mortality under hypoxia. Mol Pharm. 3(4): 424-30.
  • Solinas G, et al. (2010) JNK1 and IKKbeta: molecular links between obesity and metabolic dysfunction. FASEB J. 24(8): 2596-611.
  • Sabio G, et al. (2010) Role of the hypothalamic-pituitary-thyroid axis in metabolic regulation by JNK1. Genes Dev. 24(3): 256-64.
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