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Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, N-Flag Marqueur

    Fiche techniqueCommentairesProduits apparentésProtocoles
    Humain PTPN2 Informations sur les produits clonés de cDNA
    Gene_bank_ref_id:NM_080422.1
    Taille du ADNc:1164bp
    Description du ADNc:Full length Clone DNA of Homo sapiens protein tyrosine phosphatase, non-receptor type 2, transcript variant 2 with N terminal Flag tag.
    Synonyme du gène:PTPT, TCPTP, TC-PTP, TCELLPTP
    Espèces:Human
    Vecteur:pCMV3-N-FLAG
    Plasmid:
    Site de restriction:
    Séquence du marqueur:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Description de la séquence:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with PTPN2 qPCR primers for gene expression analysis, HP100578 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Stockage:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, N-Flag Marqueur on other vectors
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurHG10571-ACGCHF270
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurHG10571-ACRCHF270
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, N-GFPSpark MarqueurHG10571-ANGCHF270
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, N-OFPSpark MarqueurHG10571-ANRCHF270
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10571-CFCHF230
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurHG10571-CHCHF230
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurHG10571-CMCHF230
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurHG10571-CYCHF230
    Humain PTPN2/TC-PTP transcript variant 2 Gène ADNc clone le vecteur de clonageHG10571-MCHF90
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10571-M-FCHF230
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurHG10571-NFCHF230
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurHG10571-NHCHF230
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurHG10571-NMCHF230
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurHG10571-NYCHF230
    Humain PTPN2/TC-PTP transcript variant 2 expression plasmide de Gène l'ADNc ORF cloneHG10571-UTCHF230
     En savoir plus sur les vecteurs d'expression
    Product nameProduct name
    Fond

    Tyrosine-protein phosphatase non-receptor type 2, also known as T-cell protein-tyrosine phosphatase, PTPN2 and PTPT, is a cytoplasm protein which belongs to the protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase ( PTP ) family share a highly conserved catalytic motif, which is essential for the catalytic activity. TC-PTP / PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP / PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. TC-PTP / PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation, and inflammation. TC-PTP / PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.

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    Catalogue : HG10571-NF
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