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Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Flag Marqueur

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Human RAD17 Informations sur les produits clonés de cDNA
Gene_bank_ref_id:NM_133338.1
Taille du ADNc:2013bp
Description du ADNc:Full length Clone DNA of Homo sapiens RAD17 homolog (S. pombe) , transcript variant 1 with N terminal Flag tag.
Synonyme du gène:CCYC, R24L, RAD24, HRAD17, RAD17SP, FLJ41520, RAD17
Espèces:Human
Vecteur:pCMV3-N-FLAG
Plasmid:
Site de restriction:
Séquence du marqueur:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Description de la séquence:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Stockage:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Flag Marqueur on other vectors
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurHG10546-ACGCHF294
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurHG10546-ACRCHF294
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-GFPSpark MarqueurHG10546-ANGCHF294
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-OFPSpark MarqueurHG10546-ANRCHF294
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10546-CFCHF258
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurHG10546-CHCHF258
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurHG10546-CMCHF258
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurHG10546-CYCHF258
Humain Rad17 transcript variant 1 Gène ADNc clone le vecteur de clonageHG10546-MCHF90
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10546-M-FCHF258
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurHG10546-NFCHF258
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurHG10546-NHCHF258
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurHG10546-NMCHF258
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurHG10546-NYCHF258
Humain Rad17 transcript variant 1 expression plasmide de Gène l'ADNc ORF cloneHG10546-UTCHF258
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