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Humain TXN qPCR paires d'amorces

    Fiche techniqueCommentairesProduits apparentésProtocoles
    Humain TXN qPCR Product Information
    Gene_bank_ref_id:
    Synonyme du gène:TRX, TRX1, MGC61975, DKFZp686B1993, TXN
    PCR_size (bp):
    QPCR Primer Description:Verified forward and reverse primers for analyzing the quantitative expression of gene
    Contrôle de la qualité:The primer mix has been verified to generate satisfactory qPCR data on Roche LightCycler480
    Shipping_carrier:1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions) is shipped at ambiente temperatura.
    Stockage:The lyophilized product is stable for one year from date of receipt when stored at -20℃.
    The suspended product is stable for six months from date of receipt when stored at -20℃.
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    Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.

    Unique Primer Design

    To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.

    Strict Validation Process

    Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.

    Uniform PCR conditions, Saving time and cost

    ~100% amplification curve, ensuring the accuracy of the RNA quantitative

    Thioredoxin/TXN/SASP Fond

    Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein which belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

    Humain Thioredoxin/TXN/SASP Références
  • Holmgren A , et al.,1989, J Biol Chem 264 (24): 13963-6.
  • Nordberg J, et al., 2001, Free Radic Biol Med31 (11): 1287-312.
  • Mustacich D, et al., 2000, Biochem J 346 (Pt 1): 1-8. 
  • Mitchell D.A., et al., 2005, Nat. Chem. Biol. 1:154-158.
  • Size / Price
    Catalogue : HP100418
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