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Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Flag Marqueur

Fiche techniqueCommentairesProduits apparentésProtocoles
Human IL11RA Informations sur les produits clonés de cDNA
Gene_bank_ref_id:NM_004512.3
Taille du ADNc:1269bp
Description du ADNc:Full length Clone DNA of Homo sapiens interleukin 11 receptor, alpha (IL11RA), transcript variant 1 with Flag tag.
Synonyme du gène:IL11RA, MGC2146
Espèces:Human
Vecteur:pCMV2-FLAG
Plasmid:pCMV2-IL11RA-flag
Site de restriction:KpnI + XhoI (5.4kb + 1.32kb)
Séquence du marqueur:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Description de la séquence:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Stockage:The lyophilized plasmid can be stored at room temperature for three months.
pCMV2-FLAG Vector Information
 
Vector Name pCMV2-FLAG
Vector Size 5592bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag FLAG
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV2-FLAG Multiple Cloning Sites

FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Flag Marqueur on other vectors
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurHG10252-ACGCHF270
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurHG10252-ACRCHF270
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10252-CFCHF230
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurHG10252-CHCHF230
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurHG10252-CMCHF230
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurHG10252-CYCHF230
Humain IL11RA/IL-11RA transcript variant 1 Gène ADNc clone le vecteur de clonageHG10252-MCHF90
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurHG10252-M-FCHF230
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurHG10252-NFCHF230
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurHG10252-NHCHF230
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurHG10252-NMCHF230
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurHG10252-NYCHF230
Humain IL11RA/IL-11RA transcript variant 1 expression plasmide de Gène l'ADNc ORF cloneHG10252-UTCHF230
 En savoir plus sur les vecteurs d'expression
Product nameProduct name
Fond

Interleukin 11 receptor, alpha subunit (IL11RA/IL-11RA) is a subunit of the interleukin 11 receptor which is a member of the hematopoietic cytokine receptor family. IL11RA/IL-11RA is expressed in a number of cell lines, including the myelogenous leukemia cell line K562, the megakaryocytic leukemia cell line Mo7E, the erythroleukemia cell line TF1, and the osteosarcoma cell lines, MG-63 and Saos-2. It is also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain. Alternative splicing has been observed at this locus, and three variants encoding two different isoforms have been identified. IL11RA/IL-11RA is a receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. Defects in IL11RA/IL-11RA are a cause of craniosynostosis and dental anomalies (CRSDA). CRSDA is a disorder characterized by craniosynostosis, maxillary hypoplasia, and dental anomalies, including malocclusion, delayed and ectopic tooth eruption, and/or supernumerary teeth. Some patients also display minor digit anomalies, such as syndactyly and/or clinodactyly.

Références
  • Van Leuven F, et al. (1996) Molecular cloning and characterization of the human interleukin-11 receptor alpha-chain gene, IL11RA, located on chromosome 9p13. Genomics. 31 (1): 65-70.
  • Yoshizaki A, et al. (2006) Expression of interleukin (IL)-11 and IL-11 receptor in human colorectal adenocarcinoma: IL-11 up-regulation of the invasive and proliferative activity of human colorectal carcinoma cells. Int J Oncol. 29 (4): 869-76.
  • Karube K, et al. (2006) Gene expression profile of cytokines and chemokines in microdissected primary Hodgkin and Reed-Sternberg (HRS) cells: high expression of interleukin-11 receptor alpha. Ann Oncol. 17 (1): 110-6.
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