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Humain Interleukin-31 receptor A / IL31RA Gène ADNc clone le vecteur de clonage

    Fiche techniqueCommentairesProduits apparentésProtocoles
    Humain IL31RA Informations sur les produits clonés de cDNA
    Gene_bank_ref_id:BC110490
    Taille du ADNc:2295bp
    Description du ADNc:Full length Clone DNA of Homo sapiens interleukin 31 receptor A.
    Synonyme du gène:CRL, GPL, CRL3, GLMR, GLM-R, PLCA2, hGLM-R, IL-31RA, PRO21384
    Espèces:Human
    Vecteur:pGEM-T Vector
    Plasmid:pGEM-IL31RA
    Site de restriction:
    Séquence du marqueur:
    Description de la séquence:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1586 G/A resulting in the amino acid Ser substitution by Asn and 129 C/T, 2157 T/C not causing the amino acid variation.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    ( We provide with IL31RA qPCR primers for gene expression analysis, HP104467 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Stockage:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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