Mouse TWEAK / TNFSF12 HEK293 Overexpression Lysate: Product Information
This Mouse TWEAK / TNFSF12 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TWEAK / TNFSF12 protein (Cat: 50174-M01H) from the overexpression lysate was verified.
A DNA sequence encoding the mouse Tnfsf12 (NP_035744.1) (Arg105-His249) was expressed with the Fc region of human IgG1 at the N-terminus.
The recombinant mouse Tnfsf12 consists 405 amino acids and predicts a molecular mass of 44.4 kDa.
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.