Anti-Oncostatin M Antibody

Cat: 10452-RP02
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Anti-Oncostatin M Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Oncostatin M Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human Oncostatin M
Immunogen
Recombinant Human Oncostatin M / OSM protein (Catalog#10452-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Oncostatin M / OSM (rh Oncostatin M / OSM; Catalog#10452-H08H; P13725; Met 1-Arg 221). Oncostatin M / OSM specific IgG was purified by Human Oncostatin M / OSM affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Oncostatin M Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Oncostatin M Antibody (Rabbit Polyclonal antibody) Images
Human Oncostatin M/OSM Western blot (WB) 13223

Anti-OSM rabbit polyclonal antibody at 1:500 dilution

Lane A: Hu-T78 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:22 kDa

(We are unsure as to the identity of these extra bands.)

Oncostatin M Background Information

Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors typeⅠand typeⅡ both of which share the similarity of containing protein gp130 and takes part in many biometabolism processes including liver development, haematopoeisis, inflammation, bone formation and destruction and possibly CNS development. Oncostatin M (OSM) was previoustly identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6 and G-CSF, has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degree but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-teminal most 31 amino acids produces the fully active 196 residue form.

Full Name
oncostatin M
References
  • Tanaka M, et al. (2003) Oncostatin M, a multifunctional cytokine. Rev Physiol Biochem Pharmacol. Reviews of Physiology, Biochemistry and Pharmacology. 149: 39-52.
  • Auguste P, et al. (1997) Signaling of type II oncostatin M receptor. J Biol Chem. 272 (25): 15760-4.
  • Zarling JM, et al. (1986). Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells. Proc Natl Acad Sci. 83 (24): 9739-43.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Oncostatin M Antibody Rabbit Polyclonal WB,ELISA 50112-T48
    Anti-Oncostatin M Antibody Rabbit Polyclonal ELISA 50112-RP01
    Anti-Oncostatin M Antibody Mouse Monoclonal ELISA 10452-MM03
    Anti-Oncostatin M Antibody Rabbit Monoclonal ELISA 10452-R015
    Anti-Oncostatin M Antibody Rabbit Polyclonal WB,ELISA 10452-RP01
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