|Fiche technique||Commentaires||Produits apparentés||Protocoles|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CLU ORF mammalian expression plasmid, C-GFPSpark tag||RG81601-ACG|
|Rat CLU ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81601-ACR|
|Rat CLU ORF mammalian expression plasmid, C-Flag tag||RG81601-CF|
|Rat CLU ORF mammalian expression plasmid, C-His tag||RG81601-CH|
|Rat CLU ORF mammalian expression plasmid, C-Myc tag||RG81601-CM|
|Rat CLU ORF mammalian expression plasmid, C-HA tag||RG81601-CY|
|Rat CLU Gene cDNA clone plasmid||RG81601-G|
|Rat CLU ORF mammalian expression plasmid, N-Flag tag||RG81601-NF|
|Rat CLU ORF mammalian expression plasmid, N-His tag||RG81601-NH|
|Rat CLU ORF mammalian expression plasmid, N-Myc tag||RG81601-NM|
|Rat CLU ORF mammalian expression plasmid, N-HA tag||RG81601-NY|
|Rat CLU natural ORF mammalian expression plasmid||RG81601-UT|
|En savoir plus sur les vecteurs d'expression|
Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.