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Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, C-Flag Marqueur

Fiche techniqueCommentairesProduits apparentésProtocoles
Rat GALM Informations sur les produits clonés de cDNA
Gene_bank_ref_id:NM_001007704.1
Taille du ADNc:1029bp
Description du ADNc:Full length Clone DNA of Rattus norvegicus galactose mutarotase (aldose 1-epimerase) with C terminal Flag tag.
Synonyme du gène:Galm
Espèces:Rat
Vecteur:pCMV3-C-FLAG
Plasmid:
Site de restriction:
Séquence du marqueur:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Description de la séquence:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Stockage:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, C-Flag Marqueur on other vectors
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, C-GFPSpark MarqueurRG81151-ACGCHF270
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, C-OFPSpark MarqueurRG81151-ACRCHF270
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, N-GFPSpark MarqueurRG81151-ANGCHF270
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, N-OFPSpark MarqueurRG81151-ANRCHF270
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, C-Flag MarqueurRG81151-CFCHF230
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, C-His MarqueurRG81151-CHCHF230
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, C-Myc MarqueurRG81151-CMCHF230
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, C-HA MarqueurRG81151-CYCHF230
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, N-Flag MarqueurRG81151-NFCHF230
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, N-His MarqueurRG81151-NHCHF230
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, N-Myc MarqueurRG81151-NMCHF230
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF clone, N-HA MarqueurRG81151-NYCHF230
Rat GALM/Galactose Mutarotase Gène ADNc clone le vecteur de clonageRG81151-UCHF90
Rat GALM/Galactose Mutarotase expression plasmide de Gène l'ADNc ORF cloneRG81151-UTCHF230
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Catalogue : RG81151-CF
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