|Fiche technique||Commentaires||Produits apparentés||Protocoles|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TPPP3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80727-ACG|
|Rat TPPP3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80727-ACR|
|Rat TPPP3 ORF mammalian expression plasmid, N-GFPSpark tag||RG80727-ANG|
|Rat TPPP3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80727-ANR|
|Rat TPPP3 ORF mammalian expression plasmid, C-Flag tag||RG80727-CF|
|Rat TPPP3 ORF mammalian expression plasmid, C-His tag||RG80727-CH|
|Rat TPPP3 ORF mammalian expression plasmid, C-Myc tag||RG80727-CM|
|Rat TPPP3 ORF mammalian expression plasmid, C-HA tag||RG80727-CY|
|Rat TPPP3 ORF mammalian expression plasmid, N-Flag tag||RG80727-NF|
|Rat TPPP3 ORF mammalian expression plasmid, N-His tag||RG80727-NH|
|Rat TPPP3 ORF mammalian expression plasmid, N-Myc tag||RG80727-NM|
|Rat TPPP3 ORF mammalian expression plasmid, N-HA tag||RG80727-NY|
|Rat TPPP3 Gene cDNA clone plasmid||RG80727-U|
|Rat TPPP3 natural ORF mammalian expression plasmid||RG80727-UT|
|En savoir plus sur les vecteurs d'expression|
TPPP3, a member of the Tubulin polymerization-promoting protein family, is an intrinsically unstructured protein that induces tubulin polymerization. TPPP3 is a marker in the developing musculoskeletal system. In tendons, Tppp3 is expressed in cells at the circumference of the developing tendons, likely the progenitors of connective tissues that surround tendons: the tendon sheath, epitenon, and paratenon. Tppp3 is also expressed in forming synovial joints. The onset of Tppp3 expression in joints coincides with cavitation, representing a molecular marker that can be used to indicate this stage in joint transition in joint differentiation. In late embryonic stages, Tppp3 expression highlights other demarcation lines that surround differentiating tissues in the forelimb.
Depletion of TPPP3 by microRNA-based RNA interference (RNAi) inhibits cell growth, arrests cell cycles, and causes mitotic abnormalities in HeLa cells. C57BL/6 mice that received subcutaneously injected LLC (Lewis lung carcinoma) cells in which TPPP3 was knocked down showed a pronounced reduction in tumor progression. The migration/invasion activity of TPPP3-knockdown LLC cells was significantly suppressed in a transwell chamber migration assay. When these cells were injected into the tail veins of C57BL/6 mice, they exhibited milder lung metastasis compared with control tumor cells. Taken together, these findings suggested that the TPPP3 gene played an important role in tumorigenesis and metastasis, and it could potentially become a novel target for cancer therapy.