Thioredoxin 2 cDNA ORF Clone, Rat, C-DDK (Flag®) tag

Cat: RG81095-CF
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Thioredoxin 2 cDNA ORF Clone, Rat, C-DDK (Flag®) tag General Information
Gene
Species
Rat
NCBI Ref Seq
RefSeq ORF Size
501 bp
Description
Full length Clone DNA of Rat thioredoxin 2 with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Thioredoxin 2 Background Information

Thioredoxin-2, also known as TXN2, MTRX and TRX2, is a member of the thioredoxin family. Tryparedoxins (TXN) are thioredoxin-related proteins which, as trypanothione:peroxiredoxin oxidoreductases, constitute the trypanothione-dependent antioxidant defense and may also serve as substrates for ribonucleotide reductase in trypanosomatids. Thioredoxin-2 / TXN2 contains one thioredoxin domain. It is widely expressed in adult (at protein level) and fetal tissues. Human Thioredoxin-2 / TXN2 is a small redox protein important in cellular antioxidant defenses, as well as in the regulation of apoptosis. Thioredoxin-2 / TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Thioredoxin-2 / TXN2 could be involved in the resistance to anti-tumor agents. It possesses a dithiol-reducing activity. Thioredoxin-2 / TXN2 plays an important role in protecting the mitochondria against oxidative stress and in sensitizing the cells to ROS-induced apoptosis. Mammalian Thioredoxin-2 / TXN2 is a mitochondrial isoform of highly evolutionary conserved thioredoxins. Thioredoxins are small ubiquitous protein-disulfide oxidoreductases implicated in a large variety of biological functions.

Full Name
thioredoxin 2
References
  • Chen Y., et al., 2002, J. Biol. Chem. 277: 33242-8.
  • Smeets A., et al., 2005, Protein Sci. 14: 2610-21.
  • Wen,S. et al., 2009, Am J Med Genet A  149A (2): 155-60.
  • Choudhary C., et al., 2009, Science 325: 834-40.
  • Thioredoxin 2 cDNA ORF Clone, Cynomolgus, Related Products

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    Thioredoxin 2 cDNA ORF Clone in Cloning Vector, Cynomolgus CG90415-G
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    Thioredoxin 2 cDNA ORF Clone, Cynomolgus, C-His tag CG90415-CH
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    Thioredoxin 2 cDNA ORF Clone, Cynomolgus, C-HA tag CG90415-CY
    Thioredoxin 2 cDNA ORF Clone, Cynomolgus, N-HA tag CG90415-NY
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    Thioredoxin 2 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    Thioredoxin 2 cDNA ORF Clone in Cloning Vector, Rat RG81095-U
    Thioredoxin 2 cDNA ORF Clone, Rat, untagged RG81095-UT
    Thioredoxin 2 cDNA ORF Clone, Rat, C-GFPSpark® tag RG81095-ACG
    Thioredoxin 2 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG81095-ACGLN
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    Thioredoxin 2 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG81095-NF
    Thioredoxin 2 cDNA ORF Clone, Rat, C-His tag RG81095-CH
    Thioredoxin 2 cDNA ORF Clone, Rat, N-His tag RG81095-NH
    Thioredoxin 2 cDNA ORF Clone, Rat, C-HA tag RG81095-CY
    Thioredoxin 2 cDNA ORF Clone, Rat, N-HA tag RG81095-NY
    Thioredoxin 2 cDNA ORF Clone, Rat, C-Myc tag RG81095-CM
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    Thioredoxin 2 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
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    Thioredoxin 2 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG52092-ACG
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    Thioredoxin 2 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG52092-ACR
    Thioredoxin 2 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG52092-ANR
    Thioredoxin 2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG52092-CF
    Thioredoxin 2 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG52092-NF
    Thioredoxin 2 cDNA ORF Clone, Mouse, C-His tag MG52092-CH
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    Thioredoxin 2 cDNA ORF Clone, Human, Related Products

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    Thioredoxin 2 cDNA ORF Clone, Human, C-OFPSpark® tag HG12557-ACR
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    Thioredoxin 2 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG12557-CF
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    Thioredoxin 2 cDNA ORF Clone, Human, C-His tag HG12557-CH
    Thioredoxin 2 cDNA ORF Clone, Human, N-His tag HG12557-NH
    Thioredoxin 2 cDNA ORF Clone, Human, C-HA tag HG12557-CY
    Thioredoxin 2 cDNA ORF Clone, Human, N-HA tag HG12557-NY
    Thioredoxin 2 cDNA ORF Clone, Human, C-Myc tag HG12557-CM
    Thioredoxin 2 cDNA ORF Clone, Human, N-Myc tag HG12557-NM
    Thioredoxin 2 qPCR Primer Pairs, Human HP104573
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