Anti-ACVR2A Antibody

1/2

This product is not available, please contact order_us@sinobiological.com for relevant product if required.

Anti-ACVR2A Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-ACVR2A Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human ACVR2A
Immunogen
Recombinant Human ACVR2A Protein (Catalog#10257-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human ACVR2A (rh ACVR2A; Catalog#10257-H08H; NP_001607.1; Met1-Pro134). ACVR2A specific IgG was purified by Human ACVR2A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ACVR2A Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ACVR2A Antibody (Rabbit Polyclonal antibody) Images

Anti-ACVR2A rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: HeLa Whole Cell Lysate

Lane C: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:58 kDa

Observed band size:60 kDa

ACVR2A was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg HeLa Whole Cell Lysate

Lane C:0.5 mg K562 Whole Cell Lysate

2 µL anti-ACVR2A rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-ACVR2A rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the technique.

Performed under reducing conditions.

Predicted band size: 58 kDa

Observed band size :58 kDa

Anti-ACVR2A Antibody: Alternative Names

Anti-ACTRII Antibody; Anti-ACVR2 Antibody

ACVR2A Background Information

ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.
Full Name
activin A receptor, type IIA
References
  • Albertson RC, et al. (2005) Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development. Developmental dynamics 233(4): 1405-18.
  • Chung H, et al. (2008) Mutation rates of TGFBR2 and ACVR2 coding microsatellites in human cells with defective DNA mismatch repair. PloS one 3(10): e3463.
  • Fitzpatrick E, et al. (2009) Genetic association of the activin A receptor gene (ACVR2A) and pre-eclampsia. Molecular human reproduction 15(3):195-204.
  • Roten LT, et al. (2009) Association between the candidate susceptibility gene ACVR2A on chromosome 2q22 and pre-eclampsia in a large Norwegian population-based study (the HUNT study). EJHG. 17(2): 250-7.
  • Updated protocols for the detection of Sotatercept and Luspatercept in human serum
    Author
    Reichel, C;Gmeiner, G;Walpurgis, K;Thevis, M;
    Year
    2018
    Journal
    Drug Test Anal
    Application
    IP

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait