Anti-Myc tag Antibody

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Anti-Myc tag Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-Myc tag Antibody
Validated applications
WB,IP
Specificity
Myc tag
Immunogen
A synthetic Myc tag peptide conjugated to KLH
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #08
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Myc tag Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1/5000-1/10000
IP IP:1-5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Myc tag Antibody (Mouse Monoclonal antibody) Images

Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution.

Lane A: GST-myc(Recombinant protein) (5ng)

Lane B: GST-myc(Recombinant protein) (1ng)

Lane C: myc-GST(Recombinant protein) (100ng)

Lane D: myc-GST(Recombinant protein) (20ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution.

Lane A: HA-mFABP4-myc transfected 293 cell lysate (10ug)

Lane B: myc-ARG1-HA transfected 293 cell lysate (5ug)

Lane C: HA-ARG1-myc transfected 293 cell lysate (10ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

MYC was immunoprecipitated using:

Lane A:0.5 mg HA-ARG1-myc Whole Cell Lysate

Lane B:0.5 mg myc-ARG1-HA Whole Cell Lysate

4 µL anti-MYC rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-MYC rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size :40 kDa

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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