c-MET Protein, Human, Recombinant (aa 956-1390, His & GST Tag)

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c-MET Protein, Human, Recombinant (aa 956-1390, His & GST Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate.
2. Measured by its binding ability in a functional ELISA. Immobilized human HGFR (aa 956-1390) (Cat: 10692-H20B1) at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-his (Cat: 10463-H08H) with a linear range of 15.6-125 ng/ml.
Protein Construction
A DNA sequence encoding the human MET (P08581-1) (Lys956-Ser1390) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human MET /GST chimera consists of 672 amino acids and has a calculated molecular mass of 76.8 kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

c-MET Protein, Human, Recombinant (aa 956-1390, His & GST Tag): Images

c-MET Protein, Human, Recombinant (aa 956-1390, His & GST Tag): Alternative Names

AUTS9 Protein, Human; c-Met Protein, Human; DFNB97 Protein, Human; HGFR Protein, Human; RCCP2 Protein, Human

c-MET Background Information

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
Full Name
MET proto-oncogene, receptor tyrosine kinase
References
  • McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
  • Garcia S, et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-35.
  • Socoteanu MP, et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-4.
  • Kong DS, et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.
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