Zika virus (ZIKV) (strain Zika SPH2015) E / Envelope protein ( His Tag)

1/1
Price:
Size:
Number:

Zika virus (ZIKV) (strain Zika SPH2015) E / Envelope protein ( His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the Zika virus (strain Zika SPH2015) E / Envelope (ALU33341.1) (Ile291-Gly694) was expressed with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
ZIKV
Predicted N Terminal
Ile 291
Molecule Mass
The recombinant Zika virus (strain Zika SPH2015) E / Envelope consists of 415 amino acids and predicts a molecular mass of 45.5 kDa.
Formulation
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % gly.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Zika virus (ZIKV) (strain Zika SPH2015) E / Envelope protein ( His Tag): Images

ZIKV Envelope Background Information

Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
References
    1. Marina Basarab specialty registrar. et al .Zika virus. BMJ 2016; 352:i1049 doi: 10.1136/bmj.i1049
    2. Pinto Júnior VL, et al. Zika virus: a review to clinicians, Acta Med Port 2015 Nov-Dec;28(6):760-765
    3. L Zammarchi, et al.Zika virus infection in a traveler returning to Europe from Brazil, March 2015
    4. Arturo Galindo-Fraga, et al. Zika virus: A new epidemic on our doorstep. Rev Inves Clin.2015, 67: 329-32
  • Convalescent patient-derived monoclonal antibodies targeting different epitopes of E protein confer protection against Zika virus in a neonatal mouse model
    Author
    Niu, X;Zhao, L;Qu, L;Yao, Z;Zhang, F;Yan, Q;Zhang, S;Liang, R;Chen, P;Luo, J;Xu, W;Lv, H;Liu, X;Lei, H;Yi, C;Li, P;Wang, Q;Wang, Y;Yu, L;Zhang, X;Bryan, LA;Davidson, E;Doranz, JB;Feng, L;Pan, W;Zhang, F;Chen, L;
    Year
    2019
    Journal
    Emerg Microbes Infect
    Application
    cell sorting; ELISA and octet
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait