Biotinylated protein

Purification:The biotin tag can be used in affinity chromatography together with a column that has avidin (or streptavidin or Neutravidin) bound to it, which is the natural ligand for biotin.

Detection:This tag can also be used in detection of the protein via anti-biotin antibodies or avidin/streptavidin-tagged detection strategies such as enzyme reporters or fluorescent probes. This can be useful in localization by fluorescent or electron microscopy,ELISA assays, ELISPOT assays, western blots and other immunoanalytical methods. Detection with monovalent streptavidin can avoid clustering or aggregation of the biotinylated target.

Activity assay has been done on every recombinant biotinylated proteins. The assay ensure that the activity of biotinylated protein is not influenced by biotinylation.

Sino Biological Inc. has developed almost all the hot drug target proteins. A large range of biotinylated proteins can be produced as you demand.
For customization, contact [email protected].

Nearly 6000 recombinant proteins are in stock. With excellent production technology, prime cost is much lower. All the biotinylated protein are at the most favorable price. With excellent production technology, prime cost is much lower. All the biotinylated protein are at the most favorable price.

1. Viens, A.; Harper, F.; Pichard, E.; Comisso, M.; Pierron, G.; Ogryzko, V. (2008). "Use of Protein Biotinylation in Vivo for Immunoelectron Microscopic Localization of a Specific Protein Isoform". Journal of Histochemistry and Cytochemistry. 56 (10): 911–9.
2. Howarth, Mark; Chinnapen, Daniel J-F; Gerrow, Kimberly; Dorrestein, Pieter C; Grandy, Melanie R; Kelleher, Neil L; El-Husseini, Alaa; Ting, Alice Y (2006). "A monovalent streptavidin with a single femtomolar biotin binding site". Nature Methods. 3 (4): 267–73.