Loading control antibodies are used as tools to ensure equal loading of a gel during western blot and are helpful when compare the western blot results qualitatively or quantitatively. Loading control antibodies are against loading controls which are usually encoded by housekeeping genes and express stably at high levels in many cell lines and tissues. Some common loading control antibodies include Actin, Beta Actin, COX IV, GAPDH, Histone H3, Alpha Tubulin and Beta Tubulin, etc.
Sino Biological offers reliable loading control antibodies with molecular weight from 16 kDa ~ 75 kDa, for the detection of whole-cell proteins, cytoplasmic proteins, mitochondrial proteins and nucleoproteins. In addition, we also offer Transferrin antibody which can be used as loading control in serum sample.
Keep the above tips in your mind, and find the right loading control antibody for your experiments using the following table.
|Sample type||Loading control||MW(kDa)*|
|Mitochondria||VDAC1 / Porin||31|
*The listed molecular weight may vary in different species.
|Western blot analysis of GAPDH expression in 13 types of cell & tissue lysates.
Primary antibody: Anti-GAPDH mouse monoclonal antibody (Cat: 100242-MM05) at 1:500 dilution.
Secondary antibody: Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
| A: SHSY5Y whole cell lysate
B: Jurkat whole cell lysate
C: Hela whole cell lysate
D: MCF7 whole cell lysate
E: PC-3 whole cell lysate
F: Daudi whole cell lysate
G: A549 whole cell lysate
| H: Rat heart tissue lysate
I: Rat liver tissue lysate
J: Rat spleen tissue lysate
K: Rat lung tissue lysate
L: Rat kidney tissue lysate
M: Rat brain tissue lysate
|1:10,000 dilution||1:20,000 dilution||1:50,000 dilution||1:100,000 dilution|
|Western blot analysis of Beta-Tubulin expression in HepG2 (A), Hela (B) and Raw246.7 (C) whole cell lysates.
Primary antibody: Anti-Beta-Tubulin mouse monoclonal antibody (100109-MM05T) at 1:10000, 1:20000, 1:50000, 1:100000 dilution.
Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.