What are epitope tags? Epitope tags are small peptides that are fused to the protein of interest using recombinant DNA techniques. They make it possible to study recombinant proteins when protein-specific antibodies are not available or not suitable for your experimental design. Epitope tag antibodies are highly specific monoclonal and polyclonal antibodies for the detection and purification of the tagged proteins.
Sino Biological offers a full range of epitope tag antibodies with high-quality to satisfy researchers' need, including His Tag antibodies, HA Tag antibodies, Myc Tag antibodies, DYKDDDDK Tag antibodies, GFP Tag antibodies and V5 Tag antibodies, etc. All of these tag antibodies are fully validated in various applications, such as ELISA, Western Blotting (WB), Flow Cytometry (FCM), Immunoprecipitation (IP) and Immonofluorecence (IF).
Tag, tag sequences, molecular weight and corresponding epitope tag antibodies are as follows.
|Tag||Tag Sequence||Molecular Weight||Tag Antibodies|
|His Tag||HHHHHH||0.8 KD||Anti-His Tag Antibody|
|HA Tag||YPYDVPDYA||1.1 KD||Anti-HA Tag Antibody|
|Myc Tag||EQKLISEEDL||1.2 KD||Anti-Myc Tag Antibody|
|DYKDDDDK||DYKDDDDK||1 KD||Anti-DYKDDDDK (FLAG® epitope Tag) Antibody|
|V5 Tag||GKPIPNPLLGLDST||1.4 KD||Anti-V5 Tag Antibody|
|S Tag||KETAAAKFERQHMDS||1.8 KD||Anti-S Tag Antibody|
|E Tag||GAPVPYPDPLEPR||1.4 KD||Anti-E Tag Antibody|
|GFP Tag||-||26.9 KD||Anti-GFP Tag Antibody|
|EGFP Tag||-||32.7 KD||Anti-EGFP Tag Antibody|
|GST Tag||-||26 KD||Anti-GST Tag Antibody|
|Different loading quantity of His-GST-mBID transfected E.coli lysate using anti-GST epitope tag antibody (Left: Cat: 11213-MM01; Right: from Competitor A)|
|Different loading quantity of GST-3C-YWHAB transfected E.coli lysate using anti-GST epitope tag antibody (Left: Cat: 11213-MM01; Right: from Competitor A)|
|Detection of His-tagged proteins by Western Blot using Anti-His Tag epitope monoclonal antibody (Cat: 105327-MM02).
A-G: proteins with His tag at N-terminal
H-M: proteins with His tag at C-terminal
N-P: proteins with His tag at internal