SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit

COVID-19 Inhibitor Research [ FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES. ] Temporarily not available outside of China.

Intended Use

The kit applies for the screening of SARS-CoV-2 (2019-nCoV) inhibitors.

Principle of the assay

The principle of the kit is competitive ELISA. The SARS-CoV-2 (2019-nCoV) Inhibitor in the samples competes with ACE2-His (Cat: 10108-H08B) to combine with immobilized SARS-CoV-2 S Protein RBD. The signal color becomes lighter as the content of SARS-CoV-2 Inhibitor increases.

Materials Provided

Components 1 Kit (96 Tests)
SARS-CoV-2 Inhibitor (Control) 40 μL
Human ACE2 (His Tag) 1 vial
Anti-His Tag Ab (HRP) 20 μL
Microplate: Pre-coated with SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombiant Protein 1 Plate
20 × Dilution Buffer 5 mL
20 × Wash Buffer 25 mL
Color Reagent A 12.5 mL
Color Reagent B 12.5 mL
Stop Solution 8 mL

Storage

Upon receipt, please store each component at the temperature recommended in datasheet.

Typical Data

This inhibition curve is only for demonstration purposes. A curve should be generated for each assay.

SARS-CoV-2 Inhibitor (Control) concentration(nM) mean value OD450nm percent activity(%)
0 1.334  
0.004 1.311 98.2
0.012 1.303 97.7
0.037 1.283 96.2
0.110 1.312 98.3
0.329 1.245 93.3
0.988 1.150 86.2
2.963 0.839 62.9
8.889 0.242 18.1
26.667 0.059 4.4
80.000 0.052 3.9

The inhibition curve of SARS-CoV-2 Inhibitor (Control) to the binding between RBD and ACE2, IC50=3.90 nM

Application Example


Detect three monoclonal antibodies by SARS-CoV-2
Inhibitor Screening ELISA Kit (Cat# KIT001)

Citation List

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