CD23 Antibody Guideline: Preparation, Dilution and Applications

CD23 Antibody Overview

There are 24 CD23 antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, FCM, WB, ICC/IF. There are 7 CD23 antibody for ELISA, 3 CD23 antibody for IHC-P, 15 CD23 antibody for FCM, 1 CD23 antibody for WB, 1 CD23 antibody for ICC/IF. Among all these CD23 antibodies, there are 12 anti-CD23 mouse monoclonal antibodies , 8 anti-CD23 rabbit monoclonal antibodies , 4 anti-CD23 rabbit polyclonal antibodies . All the CD23 anbodies are produced in house and all are in stock. CD23 antibody customerized service is available.

CD23 Antibody ELISA

CD23 antibody ELISA preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD23 / FCER2 (rh CD23 / FCER2; Catalog#10261-H07H; P06734-1; Asp48-Ser321).

CD23 antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

CD23 Antibody western blot

CD23 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD23 / FCER2 (rh CD23 / FCER2; Catalog#10261-H07H; P06734-1; Asp48-Ser321). CD23 / FCER2 specific IgG was purified by Human CD23 / FCER2 affinity chromatography.

CD23 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CD23 Antibody flow cytometry

CD23 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD23/FCER2 (rh CD23/FCER2; Catalog#10261-H07H; P06734-1; Asp48-Ser321). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD23 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD23 Antibody immunohistochemistry

CD23 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD23 / FCER2 (rh CD23 / FCER2; Catalog#10261-H07H; P06734-1; Asp 48-Ser 321). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD23 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD23 Antibody ICC / IF

CD23 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD23 / FCER2 (rM CD23 / FCER2; Catalog#50695-M07H; P20693-1; Glu51-Pro331).

CD23 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes