Sino Biological has years of experience in antibody development and production, and is committed to providing scientific research institutions and pharmaceutical companies with one stop services including antigen production, antibody development, ELISA kit development, antibody detection and production, hybridoma antibody gene sequencing, chimeric antibody and antibody fragment expression, antibody humanization, antibody affinity maturation, anti-idiotype antibody and bispecific antibody production services.
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Antibody Development Introduction, Application Validation & How to Choose a Proper Antibody Reagent |
Download Sino Biological's newest Brochure (2019): Antibody Development: Featured Technology Platforms & Key Challenges |
Sino Biological Inc. can achieve rapid and high-throughput recombinant antibody manufacturing. The preparation cycle is only 2-6 weeks, and the monomer purity can reach 99%.
• SDS-PAGE | • SEC-HPLC >99% |
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Sino Biological Inc. has established a high-throughput mouse & rabbit monoclonal antibody R&D platform, it also has rich experience of rabbit polyclonal antibody development. All the mAb or pAb are validated by ELISA, WB, IHC, FACS et al. , and are tested by multi-tissue and multi-cell.
a)Multi-cell, multi-tissue validation |
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Fig 1. Immunochemical staining of human target E in human stomach with rabbit mAb. The image showing membrane staining of epithelium cell. |
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Fig 2. Immunochemical staining of human target E in human gastric cancer with rabbit mAb. The image showing membrane staining of epithelium cell. |
b)Multi-application verification |
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Fig 1. Immunofluorescence staining of human target E in HeLa cells with rabbit mAb. The image showing membrane staining of HeLa cells. |
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Fig 2. Lane A: A431 Whole Cell Lysate. Anti-target E rabbit mAb; Goat Anti-Rabbit IgG H&L (Dylight800). |
a)Multi-cell, multi-tissue validation |
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Fig 1. Immunochemical staining of human target D in human breast carcinoma with mouse mAb. |
b)Multi-application verification |
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Fig 1. Confocal immunofluorescence analysis of human target D in MCF7 cells. Positive staining was localized to lysosome membrane. |
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Fig 2. Flow cytometric analysis of human target D on Jurkat cells. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
a)Multi-cell, multi-tissue validation |
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Fig 1. Immunochemical staining of mouse target A in mouse liver with rabbit pAb. |
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Fig 2. Immunochemical staining of mouse target A in mouse kidney with rabbit pAb. |
b)Multi-application verification |
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Fig 1. Immunofluorescence staining of mouse target A in NIH-3T3 cells. Positive staining was localized to Mitochondrion. |
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Fig 2. Lane A: NIH-3T3 Whole Cell Lysate; Lane B: Raw264.7 Whole Cell Lysate. Anti-target A rabbit pAb; Goat Anti-Rabbit IgG (H+L)/HRP. |
Recombinant Antibody Production Service |
• Recombinant antibody production is a key technology for the industrialization of monoclonal antibody. Without immunization and screening process, antibody gene sequence can be directly & stably used to produce large amounts of antibodies. Sino Biological has a complete recombinant antibody production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers. |
Rabbit mAb Production Service |
• Professional recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on about 6000 protein production, high success rate of antigen expression |
• Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95% | |
• Leading antibody library construction and screening technology, reduce the risk of antibody fragment deletions or mismatches and increase the probability of screening for target antibodies | |
• ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity | |
• High affinity, 10-100 times more than mouse mAb, in average |
Standard Mouse mAb Production Service |
• Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95% |
• Customized immunization, screening and detection proposed according to customer's final application requirements can be provided | |
• ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity | |
• Quoting by steps, charging by nodes, reducing customer's risk and saving costs | |
• Optimized electrofusion technology has more than 3-5 times efficiency compared with PEG mediated cell fusion |
Standard Rabbit pAb Production Service |
• Professional leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on about 6000 protein production, high success rate of antigen expression |
• Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95% | |
• Serum titer ≥1:64,000 | |
• Quoting by steps, charging by nodes, reducing customer's risk and saving costs |
Fast Rabbit pAb Production Service |
• Professional recombinant protein expression and purification techniques, professional peptide design and high-efficiency conjugation techniques |
• Short production time, completing antibody production within 45 days | |
• Guaranteed rabbit pAb with a serum ELISA titer of ≥1:64,000 | |
• Antibody with high sensitivity, using less antibody each time | |
• Guaranteed delivery amount, meeting experiment needs | |
• Quoting by steps, charging by nodes, reducing customer's risk and saving costs |
Fast Mouse mAb Production Service |
• Short production time for antibodies |
• Without increasing the dose of immunogens | |
• High fusion efficiency with electrofusion technique | |
• Able to genearate antibodies for specific applications such as FC, IHC and WB | |
• Rapid production scale from milligram to gram level | |
• Antibody with high purity, high lot-to-lot reproducibility and without animal viruses contamination |
Antigen Production Service |
• International leading recombinant protein production and purification technology, with five antigen expression platforms: HEK293, CHO, E.coli, Yeast, and Insect cell, and multiple protein purification |
• With intellectual property of medium, highly efficient expression vector and high-density cell culture systems to fully improve expression success rates and save customers cost | |
• The leading technical personnel and laboratory equipment, rich experience on about 6000 protein expression and purification, supporting high-throughput, large-scale and rapid production | |
• Professional polypeptide design software, experienced designers, and efficient conjugation solutions to ensure the immunization successful rate of the designed polypeptide is greater than 95% |
ELISA Kit development Service |
• Abundant resources: 12,000 antibodies covering 2,200 targets; a recombinant protein bank of 6,000 proteins; five protein expression and production platforms including HEK293, CHO, E.coli, Yeast and Insect cell; optimized reagent formulation, enzyme labeling technology and production process; customize related products and carry out kit development according to customers' needs in least time. |
• Professional R&D platform: 500+ ELISA Kits developed in-house; rich experience on custom ELISA Kits for dozens of biopharmaceutical companies. | |
• Professional technical support: provides suitable proposal to meet customers' need fastly and efficiently. | |
• Quality assurance: sources to get various natural samples; rich testing experience; a comprehensive and accurate methodological validation of ELISA Kits. | |
• Convenient and fast: one-stop service with short timelines, high efficiency and time-saving. |