Custom Antibody Services (Production & Development)

Custom Antibody Production & Development Service Introduction

Sino Biological has years of experience in antibody development and production, and is committed to providing scientific research institutions and pharmaceutical companies with one stop services including antigen production, antibody development, ELISA kit development, antibody detection and production, hybridoma antibody gene sequencing, chimeric antibody and antibody fragment expression, antibody humanization, antibody affinity maturation, anti-idiotype antibody and bispecific antibody production services.

Antibody Development Introduction, Application Validation & How to Choose a Proper Antibody Reagent
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Antibody Development: Featured Technology Platforms & Key Challenges

Most Popular Recombinant Antibody Production Service

More Antibody Production & Development Service

  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Expression & purification
    • QC analysis
  • • Antigen preparation
    • Antigen validation
    • Immunization and serum titer test
    • Library construction & screening
    • Antibody production & purification
    • QC analysis
  • • Antigen preparation
    • Antigen validation
    • Immunization and serum titer test
    • Fusion and Screening
    • Antibody production & purification
    • QC analysis
  • • Antigen preparation
    • Immunization and serum titer test
    • Fusion and Screening
    • Antibody production & identification

Case Studies of Custom Antibody Services

A)Recombinant antibody production:

Sino Biological Inc. can achieve rapid and high-throughput recombinant antibody manufacturing. The preparation cycle is only 2-6 weeks, and the monomer purity can reach 99%.

• SDS-PAGE • SEC-HPLC >99%

B)Antibody development:

Sino Biological Inc. has established a high-throughput mouse & rabbit monoclonal antibody R&D platform, it also has rich experience of rabbit polyclonal antibody development. All the mAb or pAb are validated by ELISA, WB, IHC, FACS et al. , and are tested by multi-tissue and multi-cell.

• Rabbit mAb development service

  • a)Multi-cell, multi-tissue validation
    Fig 1. Immunochemical staining of human target E in human stomach with rabbit mAb. The image showing membrane staining of epithelium cell.
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    Fig 2. Immunochemical staining of human target E in human gastric cancer with rabbit mAb. The image showing membrane staining of epithelium cell.
  • b)Multi-application verification
    Fig 1. Immunofluorescence staining of human target E in HeLa cells with rabbit mAb. The image showing membrane staining of HeLa cells.
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    Fig 2. Lane A: A431 Whole Cell Lysate. Anti-target E rabbit mAb; Goat Anti-Rabbit IgG H&L (Dylight800).

• Standard Mouse mAb development service

  • a)Multi-cell, multi-tissue validation
    Fig 1. Immunochemical staining of human target D in human breast carcinoma with mouse mAb.
  • b)Multi-application verification
    Fig 1. Confocal immunofluorescence analysis of human target D in MCF7 cells. Positive staining was localized to lysosome membrane.
  •  
    Fig 2. Flow cytometric analysis of human target D on Jurkat cells. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

• Standard Rabbit pAb development service

  • a)Multi-cell, multi-tissue validation
    Fig 1. Immunochemical staining of mouse target A in mouse liver with rabbit pAb.
  •  
    Fig 2. Immunochemical staining of mouse target A in mouse kidney with rabbit pAb.
  • b)Multi-application verification
    Fig 1. Immunofluorescence staining of mouse target A in NIH-3T3 cells. Positive staining was localized to Mitochondrion.
  •  
    Fig 2. Lane A: NIH-3T3 Whole Cell Lysate; Lane B: Raw264.7 Whole Cell Lysate. Anti-target A rabbit pAb; Goat Anti-Rabbit IgG (H+L)/HRP.

Antibody Production & Development Service Advantages

Recombinant Antibody Production Service

• Recombinant antibody production is a key technology for the industrialization of monoclonal antibody. Without immunization and screening process, antibody gene sequence can be directly & stably used to produce large amounts of antibodies. Sino Biological has a complete recombinant antibody production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers.

Rabbit mAb Production Service

• Professional recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on about 6000 protein production, high success rate of antigen expression
• Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
• Leading antibody library construction and screening technology, reduce the risk of antibody fragment deletions or mismatches and increase the probability of screening for target antibodies
• ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
• High affinity, 10-100 times more than mouse mAb, in average

Standard Mouse mAb Production Service

• Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
• Customized immunization, screening and detection proposed according to customer's final application requirements can be provided
• ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
• Quoting by steps, charging by nodes, reducing customer's risk and saving costs
• Optimized electrofusion technology has more than 3-5 times efficiency compared with PEG mediated cell fusion

Standard Rabbit pAb Production Service

• Professional leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on about 6000 protein production, high success rate of antigen expression
• Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
• Serum titer ≥1:64,000
• Quoting by steps, charging by nodes, reducing customer's risk and saving costs

Fast Rabbit pAb Production Service

• Professional recombinant protein expression and purification techniques, professional peptide design and high-efficiency conjugation techniques
• Short production time, completing antibody production within 45 days
• Guaranteed rabbit pAb with a serum ELISA titer of ≥1:64,000
• Antibody with high sensitivity, using less antibody each time
• Guaranteed delivery amount, meeting experiment needs
• Quoting by steps, charging by nodes, reducing customer's risk and saving costs

Fast Mouse mAb Production Service

• Short production time for antibodies
• Without increasing the dose of immunogens
• High fusion efficiency with electrofusion technique
• Able to genearate antibodies for specific applications such as FC, IHC and WB
• Rapid production scale from milligram to gram level
• Antibody with high purity, high lot-to-lot reproducibility and without animal viruses contamination

Antigen Production Service

• International leading recombinant protein production and purification technology, with five antigen expression platforms: HEK293, CHO, E.coli, Yeast, and Insect cell, and multiple protein purification
• With intellectual property of medium, highly efficient expression vector and high-density cell culture systems to fully improve expression success rates and save customers cost
• The leading technical personnel and laboratory equipment, rich experience on about 6000 protein expression and purification, supporting high-throughput, large-scale and rapid production
• Professional polypeptide design software, experienced designers, and efficient conjugation solutions to ensure the immunization successful rate of the designed polypeptide is greater than 95%

ELISA Kit development Service

• Abundant resources: 12,000 antibodies covering 2,200 targets; a recombinant protein bank of 6,000 proteins; five protein expression and production platforms including HEK293, CHO, E.coli, Yeast and Insect cell; optimized reagent formulation, enzyme labeling technology and production process; customize related products and carry out kit development according to customers' needs in least time.
• Professional R&D platform: 500+ ELISA Kits developed in-house; rich experience on custom ELISA Kits for dozens of biopharmaceutical companies.
• Professional technical support: provides suitable proposal to meet customers' need fastly and efficiently.
• Quality assurance: sources to get various natural samples; rich testing experience; a comprehensive and accurate methodological validation of ELISA Kits.
• Convenient and fast: one-stop service with short timelines, high efficiency and time-saving.

Custom Antibody Production & Development Service Features

  • Varied expression platforms for antigen: Five antigen expression platforms including HEK293, CHO, E.coli, Yeast,and Insect cell can meet the needs of different types of antigen recombinant expression.
  • Multiple antigen types: Polypeptide and protein antigens are two main methods for immunization and screening, various methods such as DNA, cell and insect virus immunization are also available.
  • Various animal species for immnization: Mouse and rabbit.
  • Different antibody development technologies: Hybridoma technology and antibody library screening technology can be used to increase the probability of obtaining positive clones and high-specificity & high-affinity antibodies.
  • Diversified antibody screening technologies: Solid-phase and liquid-phase antibody screening techniques can both be used to reduce the amount of coating antigen and increase antibody diversity.
  • Multiple antibody detection platforms: ELISA, WB, IHC, FACS, SEC-HPLC, Octet, endotoxin, mass spectrometry and other antibody detection platforms can be used for comprehensive assessment of antibody quality.
  • Various antibody purification methods: Protein A, protein G, protein L, size exclusion chromatography, ion exchange chromatography, antigen affinity purification and other purification methods will be done to improve antibody purity.
  • Recombinant antibody expression technology: Using optimized plasmids and HEK293 cell line, Sino Biological enables rapid, high-throughput and large-scale production of recombinant antibodies and antibody fragments.
  • Hybridoma culture technology: Serum-free suspension culturing of hybridoma cells can be used, compared with ascites, the antibody will not include complex components and will avoid the serum influence on detection.

Contact Us

Tel
215-583-7898
Fax
+86-010-50953280