Custom Rabbit Polyclonal Antibody Production Service
Standard Rabbit pAb Production Service Introduction
Rabbit polyclonal antibody has been an important tool for scientific researchers for its advantages such as short developing time line, multiple recognition epitopes, precipitation and agglutination reactions. Sino Biological has both excellent antigen design and antibody development team, and rich experience on rabbit polyclonal antibody development. We aim to develop high-quality rabbit pAb that meets customers' application requirements.
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Standard Rabbit pAb Production Service Process
| Service procedures | Specification | Timeline | Deliverables | Guarantee | Price |
|---|---|---|---|---|---|
| ① Antigen preparationQuote！ |
Please refer to antigen production service | ||||
| ② Antigen validation |
• Analysis of client's antigen by SDS-PAGE and UV | 1-2 days | • Purified pAbs from all 30-40mL serum/rabbit • Experimental report |
ELISA titer: ≥1:64,000 | |
| ③ Immunization and serum titer test |
• Pre-immune bleed • 2 rabbits immunization • Serum titer test • Final bleed |
8-10 weeks | |||
| ④ Purification |
• Protein A purification • Antigen affinity purification (Determined by customer's request) |
3 days | |||
| ⑤ QC analysis |
• Analysis by SDS-PAGE and UV • ELISA validation |
3 days | |||
Customer-supplied antigen requirements:
| Recombinant protein antigen | Peptide antigen | ||
|---|---|---|---|
| Antigen quantities | 2.5-4mg/rabbit | • KLH/VLP conjugated peptides | 3-5mg/rabbit |
| Antigen Size | >10kD | Concentration | >0.5mg/mL |
| SDS-Page purity | >90% | • OVA/Biotin conjugated peptides | 2-3mg |
| Concentration | >0.5mg/mL | Concentration | >0.1mg/mL |
| Formulation | PBS, if not PBS, please inquire first | Formulation | PBS, if not PBS, please inquire first |
Case Studies of Standard Rabbit pAb Production Service
Sino Biological Inc. has rich experience of rabbit polyclonal antibody development, which can guarantee the serum titer of rabbit polyclonal antibody >1:64,000. All the rabbit Pab are validated by ELISA, WB, IHC, FACS et al. , and are tested by multi-tissue and multi-cell.
a）Multi-cell, multi-tissue validation
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• Mouse liver 
Fig 1. Immunochemical staining of mouse target A with rabbit polyclonal antibody. -
• Mouse kidney 
Fig 2. Immunochemical staining of mouse target A with rabbit polyclonal antibody.
b）Multi-application verification
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Fig 1. Immunofluorescence staining of mouse target A in NIH-3T3 cells. Positive staining was localized to Mitochondrion. -
Fig 2. Lane A: NIH-3T3 Whole Cell Lysate; Lane B: Raw264.7 Whole Cell Lysate. Anti-target A rabbit pAb; Goat Anti-Rabbit IgG (H+L)/HRP. -
Fig 3. Lane A: NIH3T3 Whole Cell Lysate. Anti-target A rabbit pAb; Clean-Blot IP Detection Reagent (HRP).
a）Multi-application verification
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Fig 1. Immunofluorescence staining of target B in HeLa cells. Positive staining was localized to cytoplasm and nucleus.
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Fig 2. Lane A: Hela Whole Cell Lysate; Lane B: MCF7 Whole Cell Lysate; Lane C: K562 Whole Cell Lysate. Anti-target B rabbit Pab; Goat Anti- Rabbit IgG H&L (Dylight 800).
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Fig 3. Lane A: CDK4 konckout Hela Whole Cell Lysate; Lane B: Hela Whole Cell Lysate. Anti-target B rabbit Pab; Goat Anti- Rabbit IgG H&L (Dylight 800).
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Fig 4. Lane A: HepG2 Whole Cell Lysate; Lane B: k562 Whole Cell Lysate; Lane C: Hela Whole Cell Lysate. Anti-target B rabbit Pab; Dylight 800-labeled antibody to rabbit IgG (H+L).
a）Multi-application verification
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Fig 1. Immunofluorescence staining of target C in Hela cells. Positive staining was localized to Cytoplasm.
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Fig 2. Lane A: K-562 Whole Cell Lysate. Anti-target C rabbit Pab; Goat Anti-Rabbit IgG (H+L)/HRP.
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Fig 3. Lane A: MCF7 Whole Cell Lysate; Lane B: K-562 Whole Cell Lysate; Lane C: Hep G2 Whole Cell Lysate. Anti-target C rabbit Pab; Goat Anti-Rabbit IgG (H+L)/HRP.
Standard Rabbit pAb Production Service Advantages
- State-of-the-art recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on about 6000 protein production, high success rate of antigen expression.
- Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%.
- Serum titer ≥1:64,000.
- Quoting by steps, charging by nodes, reducing customer's risk and saving costs.
